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Goat Mouse IgG1 IgG2a IgG2b IgG3 (heavy and light chains) Antibody

Purified IgG fraction of polyclonal goat antiserum to Mouse IgG, heavy and light chains. As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user's own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Typical working dilutions in histochemistry are usually between 1:100 and 1:500; in ELISA and comparable non-precipitating antibody-binding assays between 1:1,000 and 1:10,000.

Product Specifications

Reactivity

Mouse

Clonality

Polyclonal

Conjugation

Unconjugated

Source

Purified normal IgG, including all known subclasses, isolated from pooled mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Field of Research

Immunology & Inflammation; Cell Biology

Form

Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2) . No preservative added, as it may interfere with the antibody activity. No foreign protein added.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Host or Source

Goat

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