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Rabbit Mouse IgM (Fc specific) Antibody

Purified IgG fraction of polyclonal rabbit antiserum to Mouse IgM, Fc specific. As unlabelled primary or secondary antibody reagent for indirect detection of IgM in mouse cells, tissues and body fluids in immunofluorescence and immunoenzyme assay methods; for the production of immunoconjugates with a selected marker; to prepare immunoaffinity adsorbents by coupling to an artificial carrier; in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent; in Western blotting. When applied in any immunocytochemical or histochemical staining procedure or solid phase coupling techniques, the optimum concentration of this product should be established before. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:250; in ELISA and comparable non-precipitating antibody-binding assays are between 1:1,000 and 1:5,000.

Product Specifications

Reactivity

Mouse

Clonality

Polyclonal

Conjugation

Unconjugated

Source

Highly purified homogenous IgM isolated from mouse serum. Immunization with intact (19S) and split IgM (7S) . Freund’s complete adjuvant is used in the first step of the immunization procedure.

Field of Research

Stem Cells

Form

Purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2) . No preservative added, as it may interfere with the antibody activity.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Host or Source

Rabbit

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