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Rabbit Mouse IgG1 IgG2a IgG2b IgG3 IgA IgM (heavy and light chains) Antibody

Purified IgG fraction of polyclonal rabbit antiserum to mouse immunoglobulins IgG, IgA, IgM, heavy and light chains. The cytochemical grade of orb21681 allows its use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates; in radioimmunoassay; for the production of immunoconjugates with a selected marker; to prepare immunoaffinity adsorbents by coupling to an artificial carrier; in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent; in Western blotting. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:250; in ELISA and comparable non-precipitating antibody-binding assays are between 1:1,000 and 1:10,000.

Product Specifications

Reactivity

Mouse

Clonality

Polyclonal

Conjugation

Unconjugated

Source

Highly purified IgG, including all subclasses, and pools of homogenous IgA and IgM isolated from mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Field of Research

Immunology & Inflammation

Form

Purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2) . No preservative added, as it may interfere with the antibody activity.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Host or Source

Rabbit

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