Mouse NSE Protein, His Tag (MALS verified)
Mouse NSE, His Tag (NSE-M5147) is expressed from E. coli cells. It contains AA Met 1 - Met 431 (Accession # Q545V3-1) .
Product Specifications
Background
Neuron-specific enolase (NSE) is known to be a cell specific isoenzyme of the glycolytic enzyme enolase. It is also known as gamma-enolase or nolase 2 (ENO2) . It has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. NSE is a highly specific marker for neurons and peripheral neuroendocrine cells. In clinical, NSE could be used as a biomarker for neuronal injury.
Specifications
This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 49.3 kDa. The protein migrates as 47-50 kDa under reducing (R) condition (SDS-PAGE) .
Accession Number
Q545V3-1
Expression Region
Met 1 - Met 431
Host
E. coli
Target
NSE
Conjugation
Unconjugated
Tag
N-10xHis
Source
Mouse
Stability
● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin
1.0 EU per μg
Purity
95%
Bioactivity
Immobilized Mouse NSE, His Tag (Cat. No. NSE-M5147) at 1 μg/mL (100 μL/well) can bind Anti-NSE Antibody with a linear range of 0.004-0.125 μg/mL (QC tested).
Format
Powder
Buffer
20 mM Tris, 0.5 M Arginine, pH7.5
Additives
Trehalose
Molecular Weight
49.3 kDa
Shipping Conditions
RT
Storage Conditions
-20°C
Package Size
100ug*1
Product Datasheet
Https://www.acrobiosystems.com/static/main/products/pdf/ds/NSE-M5147.pdf
Species
Mouse
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