Antibacterial agent 228

Antibacterial agent 228 (Compound 8) inhibits mycobacterial ribosomes with an IC50 of 2.31 μM against Mycobacterium smegmatis. It demonstrates antibacterial activity with MIC values of 2 and 0.25 μg/mL against both wild-type and Δ1258c mutant strains of M. tuberculosis H37Rv, 8 μg/mL against wild-type and Δ2780c mutant strains of M. abscessus ATCC 19977, and 8 μg/mL against M. smegmatis.