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Mouse Human IgG (class specific) Antibody

Monoclonal mouse antiserum to human IgG, isotype specific. To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immunoperoxidase and immunofluorescence staining and immunoblotting of cytoplasmic IgG. The optimum working dilution is an assayrelated characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200; in ELISA from 1:500 upwards; in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.

Product Specifications

Reactivity

Human

Clonality

Monoclonal

Clone

NI 335 and NI 343

Conjugation

Unconjugated

Source

Highly purified IgG isolated from pooled human serum

Form

Delipidated, heat inactivated, lyophilized, stable whole ascites IgG concentration is 1.0 mg/ml. No foreign proteins added.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Host or Source

Mouse

Isotype

IgG1

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