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Mouse Human IgG4 (pFc subclass specific) Antibody

Monoclonal mouse antibody to human IgG4, subclass specific. To identify the presence of IgG4 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG4, and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200; in ELISA from 1:500 upwards; in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.

Product Specifications

Reactivity

Human

Clonality

Monoclonal

Clone

NI 315 (HP 6206)

Conjugation

Unconjugated

Source

Highly purified monoclonal IgG4 isolated from human serum

Field of Research

Immunology & Inflammation

Form

Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7.2) . No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration is 2.0 mg/ml. No foreign proteins added.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Tested Applications

ELISA, ICC, IF, IHC

Host or Source

Mouse

Isotype

IgG1

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