Mouse Human IgAc IgA1 IgA2 secretory component Antibody

Monoclonal mouse ascites containing antibodies to human IgA common determinants, IgA1, subclass specific IgA2, subclass specific and secretory components, free and bound. To identify the presence of IgA and secretory subclasses in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radioimmunoassay, ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgA, subclasses or secretory component and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly form 1:100 to 1:500; in ELISA from 1:1000 upwards; in Western blotting 1:2,000 to 1:10,000. These data should be interpreted as general recommendations only.