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TCOTP

TCOTP

Product Specifications

Product Name Alternative

TCO (1,3-diaza-2-oxophenoxazine) ribonucleoside triphosphate, Ammonium salt

Concentration

10 mM - 11 mM

Purity

≥ 95 % (HPLC)

Form

Solution in 10 mM Tris-HCl, Color: yellow

Smiles

O=P (O) (OP (=O) (OP (=O) (OC[C@@H]1[C@H] ([C@H] ([C@H] (n2cc3c (Nc4c (O3) cccc4) nc2=O) O1) O) O) O) O) O

Molecular Formula

C15H18N3O15P3 (free acid)

Molecular Weight

573.24 g/mol (free acid)

Storage Conditions

Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible

Notes

For research use only.

Applications Notes

Incorporation into RNA/cRNA - Random labeling via in vitro Transcription with T7 and SP6 RNA Polymerase [1] - 3’-End Labeling of RNA oligonucleotides with Terminal deoxynucleotidyl Transferase (TdT) [1], Spectroscopic Properties: λexc 360 nm, λem 460 nm, ε 10.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)

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