Rabbit Swine IgG (heavy and light chains), conjugated with TRITC Antibody

Tetramethylrhodamine isothiocyanate-conjugated IgG fraction of polyclonal rabbit antiserum to swine IgG, heavy and light chains. To identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates. If two Biorbyt immunoconjugates with different labels but originating from the same host animal species are used for direct two-colour staining, they can safely be mixed and used in a single step, without the danger of interaction between the two reagents. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1: 80.