Rabbit Mouse IgG2b (subclass specific), conjugated with TRITC Antibody

Tetramethylrhodamine isothiocyanate-conjugated IgG fraction of polyclonal rabbit antiserum to Mouse IgG2b, subclass specific. In immunocytochemical and immunohistochemical staining for the detection of IgG2b at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to identify and measure IgG2b in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:10 and 1:30, depending on the method used.

Product Specifications

Reactivity

Mouse

Clonality

Polyclonal

Conjugation

TRITC

Source

Pools of purified homogenous IgG2b isolated from pooled mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Form

TRITC-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2) . No preservative added, as it may interfere with the antibody activity.

Storage Conditions

The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2) . Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.