Anti Human IgG (gamma chain specific)

Anti Human IgG FITC, Low F/P - 2-4.9 - Direct Tag Antiserum to human IgG is produced in goats by immunization with purified human IgG.  The gamma globulin fraction of the absorbed, monospecific (gamma chain) antiserum is then prepared, followed by reaction with fluorescein isothiocyanate.  The excess dye is removed by dialysis and the conjugated globulin further fractionated on DEAE cellulose to remove under- and overlabelled protein.  Total protein is determined by the Biuret reaction and the concentration of fluorescein by comparisonwith a fluorescein diacetate standard.  Sodium azide (0.1% w/v) is added as preservative.