Mouse Human IgA1+IgA2 , conjugated with TRITC Antibody

Tetramethylrhodamine isothiocyanate-conjugated purified monoclonal mouse antibody to human IgA, isotype specific. To identify the presence of IgA in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as immunofluorescence staining of cytoplasmic IgA, and immunoblotting using a peroxidase labelled monoclonal antibody against TRITC. The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:20 to 1:100; in Western blotting from 1:200 upwards. Working dilutions may vary widely, strongly depending on the test conditions. These data should be interpreted as general recommendations only.