Mouse Human Bence Jones kappa (surface and hidden determinants), conjugated with TRITC Antibody

Tetramethylrhodamine isothiocyanate-conjugated purified monoclonal mouse antibody to human Ig kappa chain, free and bound. To identify the light chain type of immunoglobulins or free light chains in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as immunofluorescence staining and ELISA and immunoblotting using an indirect technique with monoclonal anti TRITC labelled with peroxidase. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:20 to 1:200; in ELISA from 1:500 upwards; in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.