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Mouse Human Bence Jones lambda (surface and hidden determinants), conjugated with Horseradish peroxidase Antibody

Horseradish peroxidase conjugated purified monoclonal mouse antibody to human Ig lambda chain, free and bound. To identify the light chain type of immunoglobulins or free light chains in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:100; in ELISA from 1:50 upwards; in Western blotting from 1:100 upwards. These data should be interpreted as general recommendations only.

Product Specifications

Reactivity

Human

Clonality

Monoclonal

Clone

NI 412, NI 268

Conjugation

HRP

Source

Highly purified Bence Jones lambda proteins isolated from pooled human urine.

Form

Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH7.2) . No preservative added, as it may interfere with the antibody activity. No foreign protein added.

Storage Conditions

The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2) . Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4°C, not refrozen, and preferably used the same day.

Notes

For research use only.

Host or Source

Mouse

Isotype

IgG1

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