Goat Human IgD (Fc specific), conjugated with Horseradish peroxidase Antibody

Horseradish peroxidase-conjugated IgG fraction of polyclonal goat antiserum to human IgD, Fc specific. In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgD at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demon- strate circulating IgD antibodies in serodiagnostic microbiology and autoimmune diseases; in non- isotopic assay methodology (e.g. ELISA) to measure IgD in human serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions may vary widely: for histochemical and cytochemical use they are usually between 1:50 and 1:500; in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:10,000.