Rabbit Sheep IgG (heavy and light chains), conjugated with Horseradish peroxidase Antibody

Horseradish peroxidase-conjugated IgG fraction of polyclonal rabbit antiserum to sheep IgG, heavy and light chains. In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG, antigen or antibody, of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure a specific IgG in sheep serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electron-dense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1: 500; in ELISA and comparable non-precipitating antibody-binding assays between 1:1,000 and 1:10,000.