Mouse Human IgG (class specific), conjugated with Horseradish peroxidase Antibody

Horseradish peroxidase conjugated purified monoclonal mouse antibody to human IgG, isotype specific. To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:500; in ELISA from 1:1000 upwards; in Western blotting from 1:2000 upwards. Working dilutions may vary widely, strongly depending on the test conditions. These data should be interpreted as general recommendations only.