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Mouse Human secretory component (free and bound), conjugated with Horseradish peroxidase Antibody

Horseradish peroxidase conjugated purified monoclonal mouse antibody to human secretory component, free and bound. To identify the presence of lactoferrin in human milk, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immuno- peroxidase and indirect immunofluorescence staining and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:20 upwards; in ELISA from 1:50 upwards; in Western blotting from 1:100 upwards. These data should be interpreted as general recommendations only.

Product Specifications

UniProt

P01833

Reactivity

Human

Clonality

Monoclonal

Clone

NI 194-4 (A89-039)

Conjugation

HRP

Source

Highly purified secretory component isolated from human milk.

Form

Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH7.2) . No preservative added, as it may interfere with the antibody activity. No foreign protein added.

Storage Conditions

The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2) . Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4°C, not refrozen, and preferably used the same day.

Notes

For research use only.

Tested Applications

DOT, ELISA, ICC, IHC, WB

Host or Source

Mouse

Isotype

IgG1

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