Mouse Human IgG2 (Fc subclass specific), conjugated with Horseradish peroxidase Antibody

Horseradish peroxidase-conjugated purified monoclonal mouse antibody to human IgG2, subclass specific. To identify the presence of IgG2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining of cytoplasmic IgG2, and immunoblotting. The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:100; in ELISA from 1:100 upwards; in Western blotting from 1:250 upwards. These data should be interpreted as general recommendations only.