AMP-PNP lithium hydrate

AMP-PNP lithium hydrate is a non-hydrolyzable ATP analog. It can substitute for ATP in biological research and is not hydrolyzed by intracellular enzymes. In a study on the vitamin B12 transporter BtuCD-F, AMP-PNP was found to block the channels formed by the two BtuCD components of the BtuCD-F complex, thereby preventing the entry of vitamin B12.