Mouse Human IgG1 (Fc subclass specific), conjugated with Horseradish peroxidase Antibody

Peroxidase conjugated purified monoclonal mouse antibody to human IgG1, subclass specific. To identify the presence of IgG1 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining of cytoplasmic IgG1, and immunoblotting. The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:40; in ELISA from 1:125 to 1:8,000; in Western blotting from 1:250 to 1:10.000. These data should be interpreted as general recommendations only.