Goat Human IgM (Fc specific), conjugated with FITC Antibody

Specificity Reference Reagent Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal goat antiserum to human IgM, Fc specific. In immunocytochemical and immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of human origin known to be of the IgM isotype in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:40 and 1:120, depending on the method used.