Cyclic-di-GMP Assay Kit

BioVision’s Cyclic-di-Guanosine Monophosphate (cyclic-di-GMP) Assay Kit is a simple, rapid, highly selective assay to measure cyclic-di-GMP levels in cells. This assay can be used to detect cyclic-di-GMP in any biochemical or enzymatic reaction that produces cyclic-di-GMP or in cell-based applications to monitor intracellular cyclic-di-GMP concentrations. In this assay, the evolution of the fluorescent signal is dependent on the initial binding of cyclic-di-GMP to the cyclic-di-GMP riboswitch in the sensor. This results in the stabilization of the SpinachTM aptamer, which in turn binds to the DFHBI-1T Fluorophore to produce a stable fluorescence, that can be measured at Ex/Em = 482/505 nm. The assay is easy to use and is homogenous. The end user needs to simply incubate the samples with the provided assay reagents for 30 min followed by reading the samples on a fluorescence plate reader at Ex/Em = 482/505 nm.