Rabbit Human IgG (Fc specific), conjugated with FITC Antibody

Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal rabbit antiserum to human IgG, Fc specific. In direct staining of cytoplasmic IgG in fixed human cells and tissue substrates; to identify circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of human in the middle layer of the test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80.