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Rabbit Monkey IgA (Fc specific), conjugated with FITC Antibody

Precipitating polyclonal rabbit antiserum to hamester immunoglobulins, heavy and light chains. Human standard Serum with assigned values for IgG subclasses. ... The determination of the concentration of each of the four subclasses was carried out by single radial immunodiffusion (RID, Mancini) in agar gel using subclass-specific polyclonal antisera and the WHO reference serum 67/97 as the primary standard. The nephelometric determination of the concentration of each of the four subclasses was carried out on a Beckman Coulter IMMAGE

Product Specifications

Reactivity

Monkey

Clonality

Polyclonal

Conjugation

FITC

Source

Pools of purified IgA isolated from Rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Form

FITC-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2) . No preservative added, as it may interfere with the antibody activity.

Storage Conditions

The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2) . Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Notes

For research use only.

Host or Source

Rabbit

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