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Goat Human IgG Fab, conjugated with FITC Antibody

Precipitating polyclonal sheep antiserum to rat Fab of IgG. In precipitating techniques as immunoelectrophoresis and single and double radial immuno- diffusion (Mancini, Ouchterlony) to identify and measure immunoglobulins; as secondary antibody to precipitate the immunoglobulin in normal rat serum.

Product Specifications

Reactivity

Human

Clonality

Polyclonal

Conjugation

FITC

Source

Purified Fab prepared from normal IgG isolated from pooled human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Field of Research

Epigenetics & Chromatin; Cell Biology

Form

FITC-coupled goat F (ab) 2 antibodies lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2) . No preservative added.

Storage Conditions

The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2) . Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Notes

For research use only.

Host or Source

Goat

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