What sizes are available for Goat Mouse IgG2 (subclass specific), conjugated with FITC Antibody?

Goat Mouse IgG2 (subclass specific), conjugated with FITC Antibody is available in the following size(s): 1 mL.

Goat Mouse IgG2 (subclass specific), conjugated with FITC Antibody

Q: What is the catalog number for Goat Mouse IgG2 (subclass specific), conjugated with FITC Antibody?

The catalog number for Goat Mouse IgG2 (subclass specific), conjugated with FITC Antibody is orb21693.

Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal goat antiserum to Mouse IgG2, subclass specific. To identify and measure IgG2, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of mouse origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80.

Product Specifications

Reactivity

Mouse

Clonality

Polyclonal

Conjugation

FITC

Source

P Pools of purified homogenous IgG2a and IgG2b isolated from mouse serum of strains belonging to the allelic types Igh-1a an Igh-1b. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Field of Research

Cell Biology

Form

Fluorochrome-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2) . No preservative added, as it may interfere with the antibody activity.

Storage Conditions

The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2) . Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.