5'-Azido-puromycin

Gee et al.[1] reported a non-radioactive alternative to analyze newly synthesized proteins in cell culture that is based on 5'-Azido-puromycin, an azide analog of puromycin. 5'-Azido-puromycin is cell-permeable and incorporates into the C-terminus of translating polypeptide chains thereby stopping translation. The resulting truncated C-terminal azide-labeled proteins can subsequently be detected via copper-free click chemistry that offers the choice to introduce a Biotin group (via DBCO-modified Biotin) for subsequent purification tasks or a fluorescent group (via DBCO-modified fluorescent dyes) for subsequent microscopic imaging.