5'-Azido-puromycin
Gee et al.[1] reported a non-radioactive alternative to analyze newly synthesized proteins in cell culture that is based on 5'-Azido-puromycin, an azide analog of puromycin. 5'-Azido-puromycin is cell-permeable and incorporates into the C-terminus of translating polypeptide chains thereby stopping translation. The resulting truncated C-terminal azide-labeled proteins can subsequently be detected via copper-free click chemistry that offers the choice to introduce a Biotin group (via DBCO-modified Biotin) for subsequent purification tasks or a fluorescent group (via DBCO-modified fluorescent dyes) for subsequent microscopic imaging.
Product Specifications
Product Name Alternative
Acetate salt
Purity
≥ 95 % (HPLC)
Form
Solid, Color: colorless
Solubility
DMSO, PBS (up to 50 mM tested) pH adjusted to 5.0
Smiles
N (=[N+]=[N-]) C[C@@H]1[C@H] ([C@H] ([C@@H] (O1) n1c2ncnc (c2nc1) N (C) C) O) NC (=O) [C@@H] (Cc1ccc (cc1) OC) N
Molecular Formula
C22H28N10O4 (free amine)
Molecular Weight
496.53 g/mol (free amine)
Storage Conditions
Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible
Notes
For research use only.
Applications Notes
Spectroscopic Properties: λ 268 nm, ε 19.5 L mmol-1 cm-1 (0.1 M HCl)
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