C8-Alkyne-dUTP

Product Specifications

Product Name Alternative

5- (Octa-1,7-diynyl) -2'-deoxyuridine 5'-triphosphate, Sodium salt

Concentration

100 mM - 110 mM

Purity

≥ 95 % (HPLC)

Form

Solution in water, Color: colorless to slightly yellow

Smiles

C1 (=O) [nH]c (=O) n (cc1C#CCCCCC#C) [C@H]1C[C@H] (O) [C@H] (O1) COP (=O) (O) OP (=O) (O) OP (=O) (O) O

Molecular Formula

C17H23N2O14P3 (free acid)

Molecular Weight

572.29 g/mol (free acid)

Storage Conditions

Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible

Notes

For research use only.

Applications Notes

Incorporation into DNA by PCR with family B polymerases (Pwo, Deep Vent exo- or KOD XL) [1,2] Incorporation into DNA by Primer Extension with family A polymerase Taq and family B polymerases (Pwo, Deep Vent exo- or KOD XL) [1] The resulting ethynyl-functionalized DNA can subsequently be processed via Cu (I) -catalyzed click chemistry that offers the choice, Spectroscopic Properties: ε292 nm 11.0 L mmol-1 cm-1 (in Tris-HCl pH 7.5)