C8-Alkyne-dCTP
C8-Alkyne-dCTP
Product Specifications
Product Name Alternative
5- (Octa-1,7-diynyl) -2'-deoxycytidine 5'-triphosphate, Sodium salt
Concentration
100 mM - 110 mM
Purity
≥ 95 % (HPLC)
Form
Solution in water, Color: colorless to slightly yellow
Smiles
C1 (nc (=O) n (cc1C#CCCCCC#C) [C@H]1C[C@H] (O) [C@H] (O1) COP (=O) (O) OP (=O) (O) OP (=O) (O) O) N
Molecular Formula
C17H24N3O13P3 (free acid)
Molecular Weight
571.31 g/mol (free acid)
Storage Conditions
Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible
Notes
For research use only.
Applications Notes
Incorporation into DNA by PCR with family B polymerases (Pwo, Deep Vent exo- or KOD XL) [1,2] Incorporation into DNA by Primer Extension with family A polymerase Taq and family B polymerases (Pwo, Deep Vent exo- or KOD XL) [1] Incorporation into DNA by 3'-End Labeling with Terminal deoxynucleotidyl Transferase (TdT) [3] The resulting ethynyl-functionalized DNA can subsequently be processed via Cu (I) -catalyzed click chemistry that offers the choice, Spectroscopic Properties: ε292 nm 9.5 L mmol-1 cm-1 (Tris-HCl pH 7.5)
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