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Mouse Human Bence Jones kappa (surface and hidden determinants), conjugated with Biotin Antibody

Biotin-conjugated purified monoclonal mouse antibody to human Ig kappa chain, free and bound. To identify the light chain type of immunoglobulins or free light chains in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:100 to 1:500; in ELISA from 1:1,000 upwards; in Western blotting from 1:2.000 upwards. These data should be interpreted as general recommendations only.

Product Specifications

Reactivity

Human

Clonality

Monoclonal

Clone

NI 285, NI 250

Conjugation

Biotin

Source

Highly purified Bence Jones kappa proteins isolated from pooled human urine.

Purification

ProteinA/G affinity purification

Form

No preservative added, as it may interfere with the antibody activity. No foreign protein added.

Storage Conditions

The lyophilized product may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2) . Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4°C, not refrozen, and preferably used the same day.

Notes

For research use only.

Host or Source

Mouse

Isotype

IgG1

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