Human FasL Protein, His-SUMO tag (Animal-Free)

Fas Ligand (FasL) is a 17.34 kDa tumor necrosis factor with 152 amino acid residues. FasL is expressed from lymphoid tissue and secreted to blood. Binding to its receptor, TNFRSF6/FAS, leads to induce apoptotic signal into cells. Involved in cytotoxic T-cell-mediated apoptosis, natural killer cell-mediated apoptosis and in T-cell development.

Product Specifications

Background

FasLis expressed from lymphoid tissue and secreted to blood. Binding to its receptor, TNFRSF6/FAS, leads to induce apoptotic signal into cells. FasL involved in cytotoxic T-cell-mediated apoptosis, natural killer cell-mediated apoptosis and in T-cell development

Product Name Alternative

Fas ligand, soluble Fas Ligand (sFasL), TNFSF6, CD95L, Apo I Ligand, APTL, APT1LG1, CD178

Gene ID

356

Accession Number

P48023

Tag

FasL

Type

Cytokines and proteins

Applications

His-SUMO tag

Purity

> 98 % as determined by SDS-PAGE

Activity

Measure by its ability to induce apoptosis in Jurkat cells. The ED₅₀ for this effect is <1 ng/mL. The specific activity of recombinant human FasL is > 1 x 10⁶ IU/mg.

Molecular Formula

29.51 kDa

Molecular Weight

29.51 kDa

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Features & Benefits

Endotoxin : < 0.1 EU per 1 μg of the protein as determined by the LAL method.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA,10% FBS or 0.1%BSA) . Please prevent freeze-thaw cycles.

Protein Length

The recombinant Human FasL Protein consists of 152 amino acids and predicts a molecular mass of 29.51 kDa.

Formulation

Lyophilized from sterile PBS, pH 8.0.

Applications Notes

Human

Other Product Names

FASL, FasL, fas ligand, soluble Fas Ligand (sFasL), TNFSF6, CD95L, Apo I Ligand, APTL, APT1LG1, CD178

Recommended Usage

Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously.