X-GAL

X-GAL (BCIG) is a widely used chromogenic β-galactosidase substrate. X-GAL is a colorless compound until cleaved by β-galactosidase, at which point X-GAL turns to an insoluble and detectable blue compound, making X-GAL particularly useful in techniques such as blue-white screening for cloning in bacteria. X-GAL can also be used for detection of β-galactosidase activity[1][2].

Product Specifications

CAS Number

[7240-90-6]

Product Name Alternative

BCIG

UNSPSC

12171500

Hazard Statement

H302, H315, H319, H335

Target

Fluorescent Dye; Glycosidase

Type

Dye Reagents

Related Pathways

Metabolic Enzyme/Protease; Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/X-GAL.html

Purity

99.88

Solubility

DMF : 200 mg/mL (ultrasonic) |DMSO : 100 mg/mL (ultrasonic)

Smiles

O[C@H]([C@@H](O)[C@H]1O)[C@@H](O[C@@H]1CO)OC2=CNC3=C2C(Cl)=C(Br)C=C3

Molecular Formula

C14H15BrClNO6

Molecular Weight

408.63

Precautions

H302, H315, H319, H335

References & Citations

[1]Sanchez-Ramos J, et al. The X-gal caution in neural transplantation studies. Cell Transplant. 2000 Sep-Oct;9 (5) :657-67.|[2]Li S, et al. In-situ SERS readout strategy to improve the reliability of beta-galactosidase activity assay based on X-gal staining in shortening incubation times. Talanta. 2021 Nov 1;234:122689.