Rabbit Bacillus subtilis a-Amylase, conjugated with Biotin Antibody
Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to alpha amylase from Bacillus subtilis. This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques) .
Product Specifications
UniProt
P00691
Reactivity
Bacteria
Clonality
Polyclonal
Conjugation
Biotin
Source
Alpha-Amylase isolated and purified from Bacillus subtilis. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Form
Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2) . No preservative added, as it may interfere with the antibody activity. No foreign protein added.
Storage Conditions
The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2) . Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
Notes
For research use only.
Tested Applications
DOT, ELISA, ICC, WB
Host or Source
Rabbit
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