Goat Mouse IgG2a (subclass specific), conjugated with Biotin Antibody

Biotin-conjugated IgG fraction of polyclonal goat antiserum to Mouse IgG2a, subclass specific. In immunocytochemical and immunohistochemical staining for the detection of IgG2a at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgG2a antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgG2a isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG2a in mouse serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user's choice has to be used This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500; in ELISA and comparable non-precipitating antibody-binding assays between 1:1.000 and 1:8,000 depending on the method used.