4,5-Diaminofluorescein

4,5-Diaminofluorescein is a fluorescent detector for nitric oxide (NO) in cells and tissues[1].

Product Specifications

CAS Number

[205391-01-1]

Product Name Alternative

DAF-2

UNSPSC

12171500

Hazard Statement

H302, H315, H319, H335

Target

Fluorescent Dye

Type

Dye Reagents

Related Pathways

Others

Field of Research

Cancer

Assay Protocol

https://www.medchemexpress.com/4-5-diaminofluorescein.html

Purity

99.34

Solubility

DMSO : 25 mg/mL (ultrasonic)

Smiles

O=C1OC2(C3=C(OC4=C2C=CC(O)=C4)C=C(O)C=C3)C5=C1C=C(N)C(N)=C5

Molecular Formula

C20H14N2O5

Molecular Weight

362.34

Precautions

H302, H315, H319, H335

References & Citations

[1]Jourd'heuil D. Increased nitric oxide-dependent nitrosylation of 4,5-diaminofluorescein by oxidants: implications for the measurement of intracellular nitric oxide. Free Radic Biol Med. 2002 Sep 1;33 (5) :676-84.|[2]Räthel TR, et al. Application of 4,5-diaminofluorescein to reliably measure nitric oxide released from endothelial cells in vitro. Biol Proced Online. 2003;5:136-142.