Human IL-33 Protein, His tag (Animal-Free)

Human IL-33 Protein, His tag (Animal-Free), expressed in E. coli

Product Specifications

Background

Interleukin 33 (IL-33) is an 18 kDa cytokine with 169 amino acid residues. Interleukin-33 is a member of the IL-1 cytokine family and is primarily expressed in endothelial cells, epithelial cells, fibroblasts, and mesenchymal cells. When IL-33 binds to its receptor, ST2L, it activates mast cells and innate lymphoid cells and subsequently generates IL-5 and IL-13, which play essential roles in allergic inflammation. IL-33 also acts as an alarm by alerting the immune system of cellular damage and plays a critical role in type-2 innate immunity.

Product Name Alternative

IL-1 F11, NF-HEV, DVS27

Accession Number

O95760

Tag

IL-33

Type

Cytokines and proteins

Applications

His tag

Purity

> 98% as determined by SDS-PAGE.

Activity

Measured by its ability to binding with recombinant ST2L (IL-33 receptor) . The ED50 for this effect is <54 ng/mL.

Molecular Weight

18.93 kDa

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Features & Benefits

Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method.

Shipping Conditions

The product is shipped with blue ice.

Storage Conditions

Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-21 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA) . Please prevent freeze-thaw cycles

Protein Length

The recombinant Human IL-33 Protein consists of 159 amino acids and predicts a molecular mass of 18.9 kDa

Formulation

Lyophilized from sterile PBS, pH 7.4.

Applications Notes

Human

Other Product Names

Human IL-33 Protein, His tag (Animal-Free), IL-33, IL-1 F11, NF-HEV, DVS27

Recommended Usage

Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously