Recombinant Xenopus tropicalis Transcription factor Sox-1 (sox1)

Product Specifications

Product Name Alternative

SRY (Sex determining region Y) -box 1

Abbreviation

Recombinant Xenopus tropicalis sox1 protein

Gene Name

Sox1

UniProt

A2TED3

Expression Region

1-401aa

Organism

Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)

Target Sequence

MYSMMMETDLHSPGVQPPSNTGQTGGGGGNKANQDRVKRPMNAFMVWSRGQRRKMAQENPKMHNSEISKRLGAEWKVMSEAEKRPFIDEAKRLRALHMKEHPDYKYRPRRKTKTLLKKDKYSLAGGLLHAAGGGHMGVGLSPGGGGGGTGGVVVQRLESPGSGASAGGYAHMNGWANGAYPGSVAAAAAAAMMQEAQLAYSQQQQQHPGSGGHHPHHHPHHPHHHPHHHPHHNPTPHPPPPPPQPMHRYDMSALQYSPLPGAQTYMTASPSGYGALGYSSSQQQHQGSPSSAAVAAAAAAAAAAAASSGALGALGSLVKSEPSVSPPVSGGGPHNRPPCPGDLREMISMYLPSGGEAGDPAAAAAAAAAAAAAATSRLHSLPQHYQGAGTGVTGTVPLTHI

Tag

N-terminal 6xHis-tagged and C-terminal 6xHis-tagged

Type

Developed Protein

Source

E.coli

Field of Research

Epigenetics and Nuclear Signaling

Relevance

Transcriptional activator. Participates in neural induction (By similarity) .

Endotoxin

Not test

Purity

Greater than 85% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Molecular Weight

44.7 kDa

References & Citations

"Sox3 acts through multiple regulatory targets to suppress Nodal signaling during Xenopus germ-layer specification." Zhang C., Grammer T.C., Basta T., Tai P., Espinosa J.M., Klymkowsky M.W. Submitted (DEC-2006)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.