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Rabbit Bovine Nucleoside 5'-diphosphate kinase, conjugated with Biotin Antibody

Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to nucleoside 5' diphos- phate kinase from bovine liver. This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immuno-fluorescence or histochemical techniques) .

Product Specifications

UniProt

P52174

Reactivity

Bovine

Clonality

Polyclonal

Conjugation

Biotin

Source

Nucleoside 5’diphosphate kinase isolated and purified from bovine liver. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Field of Research

Signal Transduction

Form

Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2) . No preservative added, as it may interfere with the antibody activity. No foreign protein added.

Storage Conditions

The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2) . Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Notes

For research use only.

Host or Source

Rabbit

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