Goat Human Ig lambda light chains (free and bound), conjugated with Biotin Antibody

Q: What is the catalog number for Goat Human Ig lambda light chains (free and bound), conjugated with Biotin Antibody?

The catalog number for Goat Human Ig lambda light chains (free and bound), conjugated with Biotin Antibody is orb21420.

Specificity Reference Reagent Biotin-conjugated IgG fraction of polyclonal goat antiserum to human Ig lambda chain, free and bound. To identify light chain type in immunocytochemical and immunohistochemical staining of immunoglobulins and to demonstrate circulating monoclonal immunoglobulins and Bence Jones proteins of the lambda type in serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user's choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions fro histochemical and cytochemical use are usually between 1:50 and 1:250; in ELISA and comparable non-precipitating antibody-binding assays between 1:1,000 and 1:5,000.

Product Specifications

Reactivity

Human

Clonality

Polyclonal

Conjugation

Biotin

Source

A pool of purified Bence Jones lambda proteins isolated from human urine. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Field of Research

Immunology & Inflammation; Cell Biology

Form

Biotin-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2) . No preservative added, as it may interfere with the antibody activity.

Storage Conditions

The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2) . Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.