Direct Blue 1

Direct Blue 1 (Chicago Sky Blue 6B) is a complex dye for background autofluorescence in immunofluorescence histochemistry. Direct Blue 1 is a potent and competitive VGLUT inhibitor. Direct Blue 1 can inhibit the Aβ-binding small molecule PrP ligand. Direct Blue 1 has anti-inflammatory activity[1][2][3][4][5][6].

Product Specifications

CAS Number

[2610-05-1]

Product Name Alternative

Chicago Sky Blue 6B

UNSPSC

12171500

Hazard Statement

H350

Target

Amyloid-β; NF-κB

Type

Dye Reagents

Related Pathways

Neuronal Signaling; NF-κB

Field of Research

Inflammation/Immunology

Assay Protocol

https://www.medchemexpress.com/direct-blue-1.html

Concentration

10mM

Purity

95.0

Solubility

DMSO : 125 mg/mL (ultrasonic) |H2O : 2.08 mg/mL (ultrasonic)

Smiles

COC1=CC(C2=CC=C(/N=N/C3=C(O)C4=C(N)C(S(=O)(O[Na])=O)=CC(S(=O)(O[Na])=O)=C4C=C3)C(OC)=C2)=CC=C1/N=N/C5=C(O)C6=C(N)C(S(=O)(O[Na])=O)=CC(S(=O)(O[Na])=O)=C6C=C5

Molecular Formula

C34H24N6Na4O16S4

Molecular Weight

992.80

Precautions

H350

References & Citations

[1]Lodha N, Poojary SA. A Novel Contrast Stain for the Rapid Diagnosis of Pityriasis Versicolor: A Comparison of Chicago Sky Blue 6B Stain, Potassium Hydroxide Mount and Culture. Indian J Dermatol. 2015;60 (4) :340-344.|[2]Yu G, et al. The antinociceptive effects of intracerebroventricular administration of Chicago sky blue 6B, a vesicular glutamate transporter inhibitor. Behav Pharmacol. 2013;24 (8) :653-658.|[3]Risse E, et al. Identification of a Compound That Disrupts Binding of Amyloid-β to the Prion Protein Using a Novel Fluorescence-based Assay. J Biol Chem. 2015;290 (27) :17020-17028.|[4]Min JO, et al. Chicago sky blue 6B inhibits α-synuclein aggregation and propagation. Mol Brain. 2022 Mar 28;15 (1) :27.|[5]Jin K, et al. Chicago sky blue 6B (CSB6B), an allosteric inhibitor of macrophage migration inhibitory factor (MIF), suppresses osteoclastogenesis and promotes osteogenesis through the inhibition of the NF-κB signaling pathway. Biochem Pharmacol. 2021 Oct;192:114734.|[6]He Z, et al. Chicago sky blue 6B, a vesicular glutamate transporters inhibitor, attenuates methamphetamine-induced hyperactivity and behavioral sensitization in mice. Behav Brain Res. 2013 Feb 15;239:172-6.