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Sheep Rabbit Glyceraldehyde-3-phosphate dehydrogenase, conjugated with Biotin Antibody

Biotin-conjugated IgG fraction of polyclonal sheep antiserum to glyceraldehyde-3- phosphate dehydrogenase from rabbit muscle. This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques) .

Product Specifications

UniProt

P46406

Reactivity

Rabbit

Clonality

Polyclonal

Conjugation

Biotin

Source

Glyceraldehyde-3-phosphate dehydrogenase isolated and purified from rabbit muscle. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Field of Research

Metabolism Research

Form

Biotin-coupled hyperimmune sheep IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2) . No preservative added, as it may interfere with the antibody activity. No foreign protein added.

Storage Conditions

The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2) . Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Notes

For research use only.

Host or Source

Sheep

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