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Rabbit Saccharomyces cerevisiae Glyceraldehyde-3-phosphate dehydrogenase, conjugated with Biotin Antibody

Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to glyceraldehyde-3- phosphate dehydrogenase from baker's yeast. This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques) .

Product Specifications

UniProt

P00359

Reactivity

Yeast

Clonality

Polyclonal

Conjugation

Biotin

Source

Glyceraldehyde-3-phosphate dehydrogenase isolated and purified from baker’s yeast. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Field of Research

Metabolism Research

Form

Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2) . No preservative added, as it may interfere with the antibody activity. No foreign protein added.

Storage Conditions

The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2) . Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Notes

For research use only.

Tested Applications

DOT, ELISA, ICC, IHC, WB

Host or Source

Rabbit

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