Recombinant Human Leptin protein (LEP) (Active)
Product Specifications
Background
May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and, or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
Product Name Alternative
Obese protein
Gene ID
3952
UniProt
P41159
Target
VPIQKVQDDT KTLIKTIVTR INDISHTQSV SSKQKVTGLD FIPGLHPILT LSKMDQTLAV YQQILTSMPS RNVIQISNDL ENLRDLLHVL AFSKSCHLPW ASGLETLDSL GGVLEASGYS TEVVALSRLQ GSLQDMLWQL DLSPGC
Purification
>97% as determined by SDS-PAGE and HPLC.
Endotoxin
Less than 1 EU/g of rHuLeptin as determined by LAL method.
Bioactivity
Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human Leptin R transfected BaF3 murine proB cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 105 IU/mg.
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Calculated Molecular Weight
16.0 kDa
Formulation
Lyophilized from a 0.2 m filtered concentrated solution in 50 mm PB, pH 3.5, with 0.02 % Tween-20.
Host or Source
E.Coli
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