2- (Methylamino) -1H-purin-6 (7H) -one

2- (Methylamino) -1H-purin-6 (7H) -one is an endogenous methylated nucleoside found in human fluids and a modified nucleoside. (In Vitro) :2- (Methylamino) -1H-purin-6 (7H) -one is the principal kinetic barrier for reverse transcription in the 1340 bases proximal to the 5' end of E. coli 16S rRNA. Transcription intermediates correspond to attenuation at the positions of 2- (Methylamino) -1H-purin-6 (7H) -one in the rRNA sequence. The relaxation time for elongation of the cDNA through m2G is approximately 3 min. 2- (Methylamino) -1H-purin-6 (7H) -one is found within both helical and looped regions of RNA secondary structure, and it can exist in either the s-cis or the s-trans rotamer. If there is a rotational preference for the methyl group, the effect of 2- (Methylamino) -1H-purin-6 (7H) -one substitution may be specific to the sequence context depending upon which face of the base participates in hydrogen bonding.