Methotrexate metabolite

Methotrexate metabolite is the active metabolite of Methotrexate which is a folic acid antagonist. Methotrexate metabolite inhibits parasite growth. (In Vitro) :Methotrexate metabolite was the most active, with 50% inhibitory concentrations in vitro of 446 ng/ml against the antifolate-sensitive strain and 812 ng/ml against the highly resistant strain under physiological folate conditions. Methotrexate metabolite potentiates the activity of the sulfone dapsone, an inhibitor of dihydropteroate synthase, but not that of chlorcycloguanil, a known inhibitor of dihydrofolate reductase (DHFR) .