N4-Acetylcytidine

N4-acetylcytidine (N4A) is an endogenous nucleoside metabolite from the degradation of tRNA. N4-Acetylcytidine is formed by N-acetyltransferase 10 and other enzymes. N4-acetylcytidine might sustain NLRP3 inflammasome activation via induction of HMGB1 expression and releasee. N4-Acetylcytidine modifies mRNA, tRNA and rRNA, affecting their stability, translation efficiency (such as enterovirus 71 RNA) . N4-Acetylcytidine is used in the study of cancer, neuroinflammatory diseases, viral infections and obesity[1][2][3][4][5].

Product Specifications

CAS Number

[3768-18-1]

UNSPSC

12352005

Hazard Statement

H302, H315, H319, H335

Target

Endogenous Metabolite; Enterovirus; Histone Acetyltransferase; NOD-like Receptor (NLR)

Type

Natural Products

Related Pathways

Anti-infection; Epigenetics; Immunology/Inflammation; Metabolic Enzyme/Protease

Applications

Metabolism-protein/nucleotide metabolism

Field of Research

Cancer; Infection; Metabolic Disease; Inflammation/Immunology; Neurological Disease

Assay Protocol

https://www.medchemexpress.com/n4-acetylcytidine.html

Purity

99.66

Solubility

DMSO : 25 mg/mL (ultrasonic; warming; heat to 60°C)

Smiles

O=C(N=C(NC(C)=O)C=C1)N1[C@@H]2O[C@H](CO)[C@@H](O)[C@H]2O

Molecular Formula

C11H15N3O6

Molecular Weight

285.25

Precautions

H302, H315, H319, H335

References & Citations

[1]Zhang H, et al. N4-acetylcytidine modifies primary microRNAs for processing in cancer cells. Cell Mol Life Sci. 2024 Feb 3;81 (1) :73.|[2]Duan J, et al. N4-acetylcytidine is required for sustained NLRP3 inflammasome activation via HMGB1 pathway in microglia. Cell Signal. 2019 Jun;58:44-52.|[3]Hao H, et al. N4-acetylcytidine regulates the replication and pathogenicity of enterovirus 71. Nucleic Acids Res. 2022 Sep 9;50 (16) :9339-9354. |[4]Wan X, et al. NAT10-mediated N4-acetylcytidine modification in KLF9 mRNA promotes adipogenesis. Cell Death Differ. 2025 Mar 23.|[5]Zhao CC, et al. NAT10-mediated mRNA N4-acetylcytidine modification of MDR1 and BCRP promotes breast cancer progression. Thorac Cancer. 2024 Apr;15 (10) :820-829.