Mouse Human IgG3 (G3m (U) allotype specific), conjugated with Biotin Antibody

Biotin-conjugated purified monoclonal mouse antibody to human IgG3m (u), allotype specific. To identify the presence of IgG3m (u) in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgG3, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer's choice have to be used. The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:100 to 1:500; in ELISA from 1:500 upwards; in Western blotting from 1:1,000 upwards. Working dilutions may vary widely, strongly depending on the test conditions. These data should be interpreted as general recommendations only.